Dr. David Muddiman
North Carolina State University
Department of Chemistry
Friday, April 14, 2023
Room 120 – Meyerhoff Chemistry Building
Host: Dr. Kamal Seneviratne
“A Novel Molecular Microscopy Platform Enabling Spatial OMICS”
Since its first demonstration in the 1960’s, the field of mass spectrometry imaging (MSI) has emerged as a fruitful area of scientific research with significant impacts to human health. To date, SIMS, MALDI, and DESI have been the primary ionization methods utilized in the field and these approaches have resulted in key new findings for a diverse range of scientific questions. However, other emerging ionization methods have great potential to impact the field of MSI. We invented matrix-assisted laser desorption electrospray ionization (MALDESI) in 2005 and over the past 17 years, we have made tremendous progress in the fundamentals, source development, and demonstrated the principal advantages of this ionization technique.
Mass spectrometry imaging offers a versatile and robust platform to discover and characterize new diagnostic, prognostic, and therapeutic biomarkers for disease, elucidate and understand pathways including protein-protein interactions, visualize endogenous and exogenous compound distributions in tissues via mass spectrometry imaging, and characterize post-translational modifications. Moreover, a Multi-OMIC approach will allow the underlying biology to be defined, enabling modeling of pathways and identify potential drug targets. This presentation will cover a diverse range of biological questions including Alzheimer’s disease, xenobiotic metabolism, cancer as well as wound healing. These approaches are made possible by innovations in chemistry and novel ionization sources developed in our laboratory. The fundamentals of these strategies will be integrated throughout the presentation.