Dr. Wayne L. Backes
Louisiana Cancer Research Center
Professor of Pharmacology
Friday, November 8, 2024
12:00 Noon
Room 120 – Meyerhoff Chemistry Building
Host: Dr. Kamal Seneviratne
“Factors governing the organization of P450 system proteins in the endoplasmic reticulum, and how this affects protein function.”
Cytochrome P450 as well as numerous other proteins coexist in the endoplasmic reticulum (ER) membrane. The relatively high concentrations of these proteins in the membrane provides numerous opportunities for the proteins to form complexes with some affecting function. Additionally, the ER membrane is not a homogeneous environment, but is organized into ordered regions enriched in cholesterol and sphingomyelin, and disordered microdomains characterized by more fluidity. Each of these factors can influence how ER proteins are organized in the membrane, and ultimately can affect protein function. This presentation will focus on two aspects of P450 system protein organization: (1) how small changes in protein structure can alter the microdomain localization of different P450 enzymes, and (2) how CYP2D6 and heme oxygenase-1 (HO-1) interact and how this affects their function.