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Lisa Kelly

Contact Information
Office: MEYR 549A
Phone: 410-455-2507
Kelly CV

Associate Professor

Ph.D. Bowling Green State University 1993; M.S. University of Rochester 1989; B.S. State University of New York at Geneseo 1988

Research Interests

Photochemical probes of macromolecular structure.

Advances in genomics and proteomics require new tools in molecular biology. In our laboratory, we are developing synthetic organic and inorganic molecules as structural probes of DNA and proteins. Through molecular engineering, we synthesize the compounds to recognize and bind to specific DNA sequences or protein residues. To date, we have used naphthalimide derivatives that are readily synthesized and functionalized. Upon activation with UV or visible light, the compounds initiate a sequence of chemical events that lead to cleavage of the macromolecule at the binding site. In parallel, transient laser spectroscopy is used to understand reaction mechanisms. Once the fragments are identified using HPLC and mass spectrometry, the DNA or protein structure may be reconstructed. Thus, these ‘photonucleases’ and ‘photoproteases’ provide new tools to identify the sequence and 3D structure of DNA and proteins.

Stimuli-Responsive Polymers.

Stimuli-responsive polymers find broad-ranging applications in smart materials and smart packaging applications.  Our group is interested in building functionalized polymers that respond, via basic photophysical phenomena, to changes in external stimuli (temperature, pressure, etc.).   Fundamentally, we are interested in gaining knowledge about how the polymer architecture governs, among other things,  the thermal properties of the polymer.  Traditional synthetic polymer chemistry is used to systematically build and functionalize the polymers.  NMR, mass spectrometry, and DSC are used to characterize the materials.  Steady-state and time-resolved fluorescence methods are used to understand the fundamental photophysics.  Specifically, using time-correlated single-photon counting, the polymer dynamics and rate constants for excited-state interconversions, are mapped out to understand which elementary reaction step controls the temperature-dependent photophysics.  As a result, systematic structure-function correlations are obtained.


Selected Publications

  1. Sova, Stacey and Kelly, Lisa A.; “Formation and Reaction Kinetics of Biradicals and Triplet States in a Series of Carboxylated 1,4,5,8-Naphthalene Diimides,” J. Phys. Chem. A, 2020, Just Accepted Manuscript,
  2. Hopanna, Mamatha; Kelly, Lisa A.; Blaney, Lee “Photochemistry of the Organoselenium Compound Ebselen:  Direct Photolysis and Reaction with Active Intermermediates of Conventional Reactive Species, Sensitizers and Quenchers,” Env. Sci. Tech, 2020, Just Accepted Manuscript,
  3. Setera, Brett, Arnold, Bradley, Choa, Fow-Sen, Kelly, Lisa, Emge, Ian, Su, Charmain, Machuga, Krishna S., Singh, N.B. “Design and Measurements of n for the Multicomponent Semiconductor,” Proc. SPIE, 2020, 11498, 11498, 1149803-1 -1149803-7.
  4. Emge, Ian; Su, Charmain; Sood, Rachit; Arnold, Bradley; Choa, Fow-Sen; Kelly, Lisa; Mandal, KD; Verma, Manish K; Singh, NB “Chemical and Biological Sensing Using Polarity of Material,” Proc. SPIE2020, 11416, 1141610-1 – 1141610-6.
  5. Singh, Narsingh B; Su, Ching-Hua; Choa, Fow-Sen; Arnold, Bradley; Cooper, Christopher; Cullum, Brian; Kelly, Lisa “Morphology and Performance of Organic Nanocomposites for γ-Ray Sensing,” Emerging Mater. Res., 2020, 9, 1 – 7.
  6. Sova, S. and Kelly, Lisa A. “Enzyme Modification and Oxidative Crosslinking using Carboxylate-, Phenol- and Catechol-Conjugated 1,8-Naphthalimides,” Photochemi. Photobiol., 2019, 95, 1169 – 1178. doi: 10.1111/php.13110
  7. Sova, Stacey; Prasad, Narasimha; Cooper, Christopher; Kelly, Lisa; Arnold, Bradley; Cullum, Brian; Choa, Fow-Sen; Singh, NB. “Importance of Lotus Effect on Surface Sensing,” Proc. SPIE, 2019, 11020, 1102005-1 – 1102005-7. doi: 10.1117/12.2519738
  8. McAdams, Joel; Bowman, Eric; Cullum, Brian; Arnold, Bradley; Kelly, Lisa; Choa, Fow Sen; Singh, NB; Su, Ching Hua; Mandal, KD; Singh, Shruti “Effect of Processing on Morphology of Hydroxyapatites: Bioactive Glasses and Crystalline Composites,”  Proc. SPIE, 2019, 11020, 1102006-1 – 1102006-9. doi: 10.1117/12.2516042
  9. AndersonErin D.; SovaStacey; IvanicJoseph; Kelly, Lisa; Schnermann, Martin J. “Defining the Conditional Basis of Silicon Phthalocyanine Near-IR Ligand Exchange,”  Phys. Chem. Chem. Physi., 2018, 20, 19030 – 19036. doi: 10.1039/c8cp03842b
  10. Singh, N. B.; Su, Ching Hua; Arnold, Bradley; Choa, Fow-Sen; Cooper, Christopher; Sova,Stacey; Gill, Puneet; Dayal, Vishall; Kelly, Lisa, Prasad, Narasimha; Smith,Paul; Cullum, Brian “Effect of Additives: Organic-Metal Oxide Nanocomposites for γ-ray Sensors,” Proc. SPIE, 2018, 10629, 106290D-1 – 106290D-6. doi: 10.1117/12.2301084
  11. Bhavsar, Jayati ; Tufail, Areeba; Gautam, Pooja; Arnold, Brad; Choa, Fow-Sen; Kelly, Lisa; Cullum, Brian; Smith, Paul;  Su, Ching Hua; Mandal, K. D.; Singh, N. B. “Design and Characteristics of Hydroxyapatites: Effect of Radiation.” Proc. SPIE, 2018, 10662, 1066205-1 – 1066205-6. doi: 10.1117/12.2301032
  12. Prasad, Narasimha S.; Sova, Stacey; Kelly, Lisa; Bevan, Talon; Arnold, Bradley; Cooper, Christopher; Choa, Fow-Sen ; Singh, N. B. “Optical Characteristics of Novel Bulk and Nanoengineered Laser Host Materials,” Proc. SPIE, 2018, 10533, 1053320-1 – 1053320-7. doi: 10.1117/12.2295994
  13. Razdan, V.; Singh, A.; Arnold, A.; Choa, F-S.; Kelly, L.; Singh, N. B. “Effect of Organic Flux on the Colossal Dielectric Constant of CaCu3Ti4O12 (CCTO),” Proceedings of SPIE, 2015, 9493, 949308-1 – 949308-5.
  14.  Manning, S. J.; Bogen, W.; Kelly, L. A. ‘Synthesis, Characterization, and Photophysical Study of of Fluorescent N-Substituted Benzo[ghi]perylene ‘Swallow Tail’ Monoimides,’ The Journal of Organic Chemistry201176, 6007-6013.
  15. Trexler, M. M.; Zhang, D.; Kelly, L.; Sample, J. ‘Crystal Structure and Optical Properties of Erbium- and Neodymium-Doped Zirconia Nanoparticles,’ Journal of Materials Research201025, 500 – 509.
  16. Arnold, B.; Kelly, L.; Oleske, J. B.; Schill, A. ‘Standoff Detection of Nitrotoluenes Using 213-nm Amplified Spontaneous Emission from Nitric Oxide,’ Analytical and Bioanalytical Chemistry, 2009395, 349-355.
  17. Breidenich, Jennifer; Patrone,Julia; Kelly, Lisa; Benkoski, Jason; Le, Huong; Sample, Jennifer “Chemiluminescent Solid Lipid Nanoparticles (SLN) and Interations with Intact Skin,” Proceedings of SPIE20097397, 73970L-1 – 73970L-10.
  18. Schill, Alexander W.; Arnold, Bradley R.; Kelly, Lisa A.; Pellegrino, Paul, M. ‘Standoff Detection using Coherent Backscattered Spectroscopy,’ Proceedings of SPIE20076554, 6554G-1 – 6554G-8.
  19. McMasters, S. and Kelly, L. A. ‘Sequence-Dependent Interactions of Cationic Naphthalimides and Polynucleotides,’ Photochemistry and Photobiology200783, 889 – 896.
  20. McMasters, S.; Kelly, L. A. “Ground-State Interactions of Spermine-Substituted Naphthalimides with Mononucleotides,” Journal of Physical Chemistry, B, 2006110, 1046-1055.
  21. Kimaro, A.; Kelly, L. A.; Murray, G. M., “Synthesis and Characterization of Molecularly Imprinted Uranyl Ion Exchange Resins,” Separation Science and Technology200540, 2035–2052.
  22. Abraham, B.; McMasters S.; Mullan, M. A.; Kelly, L. A., ‘Reactivities of Carboxy-Substituted 1,4,5,8-Naphthalene Diimides in Aqueous Solution,’ Journal of the American Chemical Society2004126, 4293-4300.
  23. Chandrasekharan, N; Kelly, L. A., ‘Progress Towards Fluorescent Molecular Thermometers,’ in Reviews in Fluorescence 2004, Geddes, C. D., Editor, 2004, pgs. 21-40.
  24. Abraham, B.; Kelly, L., ‘Photo-oxidation of Amino Acids and Proteins Mediated by Novel 1,8-Naphthalimide Derivatives,’ Journal of  Physical Chemistry B 2003107, 12534 – 12541.
  25. Rogers, J. E.; Le, T. P.; Kelly, L. A., ‘Nucleotide Oxidation Mediated by Naphthalimide Excited States with Covalently Attached Viologen Cosensitizers,’ Photochemistry and  Photobiology 200173, 223-229.
  26. Kimaro, A.; Kelly, L. A.; Murray, G. M., ‘Molecularly Imprinted Ionically Permeable Membrane for Uranyl Ion,’ Chemical Communications 2001, 1282 – 1283.
  27. Rogers, J. E.; Abraham, B.; Rostkowski, A.; Kelly, L. A., ‘Mechanisms of Photoinitiated Cleavage of DNA by 1,8-Naphthalimide Derivatives,’ Photochemistry and Photobiology 200174, 521-531.
  28. Chandrasekharan, N. and Kelly, L. A. ‘A Dual Fluorescence Temperature Sensor Based on Perylene/Exciplex Interconversion,’ Journal of the American Chemical Society 2001123, 9898-9899.
  29. Rogers, J. E.; Weiss, S. J.; Kelly, L. A., ‘Photoprocesses fo Naphthalene Imide and Diimide Derivatives in Aqueous Solutions of DNA,’ Journal of the American Chemical Society 2000122, 427-436.
  30. Le, T. P.; Rogers, J. E.; Kelly, L. A., ‘Photoinduced Electron Transfer in Covalently Linked 1,8-Naphthalimide/Viologen Systems,’ Journal of Physical Chemistry A. 2000104, 6778-6785.
  31. Rogers, J. E.; Kelly, L. A., ‘Nucleic Acid Oxidation Mediated by Naphthlene and Benzophenone Imide and Diimide Derivatives: Consequences for DNA Redox Chemistry,’ Journal of the American Chemical Society 1999121, 3854-3861.

Honors and Awards

Elected President
American Society for Photobiology
2005 – 2006
“The Fluorescence Omnilyzer”
R&D 100 Award
DOE Distinguished Post-Doctoral Fellow
Department of Energy – ORISE
1993 – 1996


A Photophysical Study of Stimuli-Responsive Polymers
NSF CHE-0415429
Fluorescent Polymers as Temperature-Responsive Smart Coatings
Rohm and Haas
Stand-Off Detection of Energetic Materials Using Back-Scattered Coherent Spectroscopy
Johns Hopkins Applied Physics Laboratory
Arnold, B. A. and Kelly, L. A. co-PIs


Courses Taught

  • CHEM 303: Physical Chemistry for Biochemical Sciences
  • CHEM 302: Physical Chemistry II
  • CHEM 420: Computer Applications in Chemistry
  • CHEM 443: Molecular Spectroscopy and Biomacromolecules
  • CHEM 490: Special Topics in Chemistry – Applications of Photochemistry and Spectroscopy.
    This course focuses on the fundamental aspects of photophysical and photochemical phenomena. Fundamental aspects of the creation and fate of electronically excited states are covered. The application of modern instrumentation to probe excited-state dynamics are emphasized. Topics include the theory and practical aspects of fluorescence and transient absorption spectroscopies. The course emphasizes the interaction of light with molecular systems to (i) initiate and understand important light-initiated reactions, including photosynthesis, vision, and nucleic acid photochemistry and (ii) develop practical sensors for use in biological and environmental applications. Prequisites: CHEM 302 or 303 and CHEM 352 or equivalent. NOTE: This course is also offered at the 600 level for graduate students.
  • CHEM 499: Undergraduate Research
  • CHEM 399: Tutorial Projects in Chemistry
  • CHEM 420: Computer Applications in Chemistry


Research Description

fluorescent synthesis
 Fluorescent dye synthesis (Yu and Matthew)
Laser Flash Photolysis
               Nanosecond laser flash photolysis

Photophysical Studies of Stimuli-Responsive Polymers.

Our group is broadly interested in fabricating and studying new polymers that have utility as stimuli-responsive materials.  These materials are made by co-polymerizing a fluorescent dye with a variety of styrene or acrylamide co-monomers.  Fundamental photophysics are used to tailor these materials for a response of choice.   For example, to map temperature distributions in real time on any surface or object, we are fabricating polymeric coatings that exhibit exciplex or excimer formation.  This phenomenon is characterized by a two-color emission.  By imaging the intensities of each color, as a function of spatial coordinate, the precise temperature on each point of the object can by quantified and monitored in real time.  At the same time, we are interested in developing polymeric coatings that exhibit a time-temperature response for smart packaging applications (like when has your milk jug been left too warm too long!).  These materials take advantage fluorescence quenching, which can be thermally reversed via a chemical reaction in the polymer (i.e. your milk jug lights up under black light when it has risked spoiling!).

Photochemical Probes of Macromolecular Structure.

Stacey-2014-1Complementary methods of transient laser spectroscopy and traditional bioanalytical methods are used to investigate the photocleavage and photomodification of proteins and DNA.  Research projects begin with  the synthesis of functionalized aromatic imides and diimides designed to photoinduce a specific kind of damage (cleavage, cross-linking, affinity labeling, etc.).   Next, the photophysical and redox properties are assessed using transient  and steady-state spectroscopy, along with electrochemical methods.  Transient absorption spectroscopy is used to identify the early photochemical events with viable targets (nucleic acids and peptides or proteins).  Finally, traditional bioanalytical methods are used to correlate the early photochemical events with the modified macromolecules.  The complementary approach offers a fundamental understanding into the development of new small molecules that can be light-activated and serve as static and dynamic probes of macromolecular structure.