Dr. Craig Cameron
University of North Carolina – Chapel Hill
Friday, March 28, 2025
12:00 Noon
Room 120 – Meyerhoff Chemistry Building
Host: Dr. Aaron Smith
“Complexities of the Enterovirus Lifecycle Revealed by Analysis of Single Cells and Cell Pairs”
Viruses encounter numerous bottlenecks in moving from the primary site of infection to sites that are responsible for disease. Establishment of a productive infection requires the viral population to survive these bottlenecks. What this means practically is that a virus population requires the ability to withstand multiplicities of infection (MOIs) far lower than those used for a one-step-growth analysis, an experimental condition in which genetic complementation can even suppress lethal mutations. We have developed microfluidics-based approaches to study viral infection dynamics and cell pairs at a range of MOIs. We will share some of the fascinating observations made using our single-cell and cell-pairing methods, including our recent discovery of a viral non-structural protein capable of hijacking cellular secretory macroautophagy pathway to promote non-lytic spread of an enterovirus.