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Brian Cullum

Contact Information
Office: MEYR 243B
Phone: 410-455-2833
Cirriculum Vitae: Cullum_CV
Lab Website

Associate Professor

Post-Doctoral Oak Ridge National Laboratory 1999; Ph.D. University of South Carolina 1998; B.S. Frostburg State University 1994

Professional Interests

Bioanalytical and Environmental Spectroscopy

Research in our group involves the development of novel sensing tools and strategies for the monitoring of biological and environmental systems at both the nano- and macro-scales.  On the nano-scale, we are developing implantable sensors capable of being inserted into individual cells and optically positioned for the monitoring of cellular response to various external stimuli.  On the macro-scale, we are developing new spectroscopic techniques for real-time disease (i.e., cancer) diagnosis in clinical and surgical settings.

Selected Publications

B. M. Cullum, “Sample Collection and Preparation of Liquids and Solids,” in Handbook of Spectroscopy; Gunter Gauglitz and Tuan Vo-Dinh (eds.), Weinheim, Germany, Wiley-VCH Verlag GmbH & Co., 2nd edition, 2011, in press.
T. Vo-Dinh and B. M. Cullum, “Fluorescence Spectroscopy for Biomedical Diagnostics,” in CRC Handbook of Biomedical Photonics; Tuan Vo-Dinh (ed.), Boca Raton, CRC Press, 2nd edition, 2011, in press.
C. K. Klutse, T. Vo-Dinh and B. M. Cullum , “Nanosensors for Single-Cell Analyses,” in CRC Handbook of Biomedical Photonics; Tuan Vo-Dinh (ed.), Boca Raton, CRC Press, 2nd edition, 2011, in press.
C. K. Klutse, and B. M. Cullum, “Development of SAM-based Multi-layer SERS Substrates for Intracellular Analyses”, Proc. SPIE – Soc. Opt. Eng.7674, p. 767402-1 – 767402-12 (2010).
J. B. Kiser and B. M. Cullum, Optical Cross-talk and Surface Characterization of SERS Nanoimaging Bundle Substrates:, Proc. SPIE – Soc. Opt. Eng.7674, p. 76740D-1 – 76740D-7 (2010).
B. M. Cullum, “Sub-Diffraction Limited Raman Chemical Imaging,” in Applications of Surface-Enhanced Raman Spectroscopy; Stuart Farquharson (ed.), New York, CRC Press, in press 2009.
C. K. Klutse, H. Li and B. M. Cullum, “Optimization of Multilayer Surface-enhanced Raman Scattering (SERS) Immuno-nanosensors via Self-assembled Monolayer Spacers,” Proc. SPIE – Soc. Opt. Eng.7313, p. 73130C1 – 73130C10 (2009).
J. B. Kiser, M. E. Hankus and B. M. Cullum, “Tunable Fiber Optic Imaging Bundle SERS Substrates,” Proc. SPIE – Soc. Opt. Eng.7313, p. 73130E1 – 73130E8 (2009).
J. Sun, M. E. Hankus and B. M. Cullum, “SERS-based Immuno-microwell Arrays for Multiplexed Detection of Food-borne Pathogenic Bacteria,” Proc. SPIE – Soc. Opt. Eng.7313, p. 73130K1- 73130K10 (2009).
B. M. Cullum*, H. Li, M. V. Schiza and M. E. Hankus, “Characterization of Multi-Layer Enhanced SERS Substrates and Their Potential for SERS Nano-Imaging,” Nanobiotechnol., 3 (1), p. 1 – 11,  (2007).
H. Li, C. E. Baum, J. Sun and B. M. Cullum, “Multilayer Enhanced Gold Film Over Nanostructure (GFON) Surface-Enhanced Raman Substrates,” Appl. Spectrosc. 60 (12), p. 1377 – 1386,  (2006).
M. E. Hankus, G. J. Gibson and B. M. Cullum, “Surface Enhanced Raman Scattering (SERS)-based Nanoprobe for High Resolution, Non-scanning Chemical Imaging,” Anal. Chem. 78 (21), p. 7535 – 7546, (2006).
H. Li, C. E. Baum and B. M. Cullum, “Characterization of Novel Gold SERS Substrates with Multilayer Enhancements,” SPIE Proceedings, 6380, p. 63800D1 – 63800D12 (2006).
M. E. Hankus and B. M. Cullum, “SERS Probes for Imaging Individual Biochemical Species on the Nanoscale,” SPIE Proceedings, 6380, p. 638004/1 – 638004/12 (2006).
H. Li, J. Sun and B. M. Cullum, “Label-Free Detection of Proteins Using SERS-Based Immuno-Nanosensors,”Nanobiotechnol., 2 (1-2), p. 17 – 28 (2006).
H. Li, C. E. Baum, J. Sun and B. M. Cullum, “Multilayer Enhanced SERS Active Materials: Fabrication, Characterization, and Application to Trace Chemical Detection,” SPIE Proceedings , 6218, p. 22-33, (2006).
H. Li and B. M. Cullum, “Dual Layer and Multilayer Enhancements from Silver Film over Nanostructured Surface-Enhanced Raman Substrates,” Appl. Spectrosc. 59 (4), p. 410 – 417, (2005).
J. B. Kiser, N. Chandrasekharan and B. M. Cullum, “Sub-surface Brain Tumor Diagnosis Using a Portable NMPPAS-based Fiber Optic Microprobe,” SPIE Proceedings, 6007, p.133-142, (2005).
H. Li., C. E. Baum, J. Sun and B. M. Cullum, “Label-free Detection of Antigens using Implantable SERS Nanosensors,” SPIE Proceedings, 6007, p. 58-70, (2005).
M. E. Hankus, G. J. Gibson and B. M. Cullum, “Characterization and Optimization of Novel Surface-Enhanced Raman Scattering (SERS)-based Nanoimaging Probes for Chemical Imaging,” SPIE Proceedings,6007, p. 9 – 20, (2005).
B. M. Cullum, “Nanoscale Optical Biosensors and Biochips for Cellular Diagnostics,” in Smart Biosensor Technology; George K. Knopf and Amarjeet S. Bassi (eds.) Boca Raton, CRC Press, (2006) pp. 109 – 133.
H. Li, J. Sun, T. A. Alexander and B. M. Cullum, “Implantable SERS Nanosensors for Pre-symptomatic Detection of BW (Biological Warfare) Agents,” SPIE Proceedings, 5795, p. 8-18 (2005).
M. B. Wabuyele, M. E. Martin, F. Yan, D. L. Stokes, J. Mobley, B. M. Cullum, A. L. Wintenberg, R. Lenarduzzi and T. Vo-Dinh, “Portable Raman Device for Detection of Chemical and Biological Warfare Agents,” SPIE Proceedings, 5692, p. 330 – 336, (2005).
N. Chandrasekharan, J. Kiser and B. M. Cullum, “Development of a Portable Multiphoton Photo-acoustic Spectroscopy System for Tumor Diagnostics,” SPIE Proceedings, 5588, p.169-178, (2005).
M. Hankus, G. Gibson, N. Chandrasekharan and B. M. Cullum, “Surface Enhanced Raman Scattering (SERS) – Nanoimaging Probes for Biological Analysis,” SPIE Proceedings, 5588, p.106-117, (2005).
H. Li, P. Patel and B. M. Cullum, “Novel multilayered SERS substrates for trace chemical and biochemical analysis,” SPIE Proceedings, 5588, p.87-98, (2005).
H. Li, J. Sun and B. M. Cullum, “Nanosphere-based SERS immuno-sensors for protein analysis,” SPIE Proceedings, 5588, p.19-31, (2005).
N. Chandrasekharan, B. Gonzales and B. M. Cullum, “Non-Resonant Multiphoton Photoacoustic Spectroscopy for Non-invasive Subsurface Chemical Diagnostics,” Appl. Spectrosc. 58 (11), p.1325-1333 (2004).
B. M. Cullum, “Optical Nanosensors and Nanobiosensors,” in Encyclopedia of Nanoscience and Nanotechnology; New York, Marcel Dekker, Inc., (2004), pp. 2757 – 2768.
H. Li and B. M. Cullum, “Development and Characterization of SERS-based Immuno-Nanosensors for Single Cell Analyses,” SPIE Proceedings, 5261, p.142-154, (2004).
N. Chandrasekharan, R. Mehta, S. Saha and B. M. Cullum, “Development of Multiphoton Photoacoustic Spectroscopy for Non-invasive Tissue Diagnostics,” SPIE Proceedings, 5261, p.58-69, (2004).
J. Mobley, B. M. Cullum, A. L. Wintenberg, S. S. Frank, R. A. Maples, D. L. Stokes and T. Vo-Dinh, “Single-board Computer Based Control System for a Portable Raman Device with Integrated Chemical Identification,” Rev. Sci. Instrum., 75 (6), p. 1-8 (2004).
T. Vo-Dinh, B. M. Cullum and P. Kasili, “Development of a Multi-spectral Imaging System for Medical Applications,” J. Phys. D. – Appl. Phys., 36 (14), p. 1663-1668 (2003).
L. R. Allain, D. N. Stratis-Cullum, B. M. Cullum, J. Mobley, M. R. Hajaligol and T. Vo-Dinh, “Real-time Detection of PAH Mixtures in the Vapor Phase at High Temperatures,” J. Anal. Appl. Pyrolysis, 66, p. 145 – 154 (2003).
B. M. Cullum and T. Vo-Dinh, “Nanosensors: Development and Application of Nanosensors for Cellular Diagnostics,” in CRC Handbook of Biomedical Photonics; Boca Raton, CRC Press, (2003), pp. 60-1 – 60-20.
T. Vo-Dinh and B. M. Cullum, “Fluorescence Spectroscopy: Instrumentation and Application to Tissue Diagnostics,” in CRC Handbook of Biomedical Photonics; Boca Raton, CRC Press, (2003), pp. 28-1 – 28-50.
D. N. Stratis-Cullum, D. L. Stokes, B. M. Cullum, J. M. Song, P. M. Kasili, R. Jagannathan, J. Mobley and T. Vo-Dinh, “Spectroscopic Data in Biologically and Medically Relevant Species and Samples,” in CRC Handbook of Biomedical Photonics; Boca Raton, CRC Press, (2003), pp 65-1 – 65-136.
B. M. Cullum and T. Vo-Dinh, “Sensors for Biological Applications: Spectroscopic Techniques at the Cellular Level,” in Recent and Evolving Advanced Semiconductor and Organic Nano-technologies; Academic Press, Volume 3, (2003), pp. 225 – 250.
B. M. Cullum and T. Vo-Dinh, “Sample Collection and Preparation of Liquids and Solids,” in Handbook of Spectroscopy; Weinheim, Germany, Wiley-VCH Verlag GmbH & Co., (2003), pp. 17-35.
J. M. Song, B. M. Cullum, J. Mobley, J. S. Bogard, M. Moscovitch, G. W. Phillips and T. Vo-Dinh, “Crossed-beam two-photon Readout System for Three-dimensional Radiation Dosimeters,” Rev. Sci. Instrum., 73(12), p. 4214-4217 (2002).
M. Moscovitch, G. W. Phillips, B. M. Cullum, J. Mobley, J. S. Bogard, D. Emfietzoglou and T. Vo-Dinh, “Radiation Dosimetry Using Three-dimensional Optical Random Access Memories,” Radiat. Protection Dosimetry, 101 (1-4), p. 17-22 (2002).
P. M. Kasili, B. M. Cullum, G. D. Griffin and T. Vo-Dinh, “Nanosensor for In vivo Measurement of the Carcinogen Benzo [a] Pyrene in a Single Cell,” J. Nanosci. Nanotech., 2 (6), p. 653-658 (2002).
B. M. Cullum, J. Mobley, D. L. Stokes, L. R. Allain, D. N. Stratis, A. L. Wintenberg, R. A. Maples and T. Vo-Dinh, ‘Development of a Portable Raman Spectrometer for Medical Diagnostics,’ SPIE Proceedings, 4615, p.82-90, (2002).
B. M. Cullum, G. D. Griffin and T. Vo-Dinh, ‘Nanosensors: Design and Application to Site-Specific Cellular Analyses,’ SPIE Proceedings, 4615, p.148-154, (2002).
T. Vo-Dinh, B. M. Cullum, J. Mobley and P. M. Kasili, ‘Multispectral Imaging for Medical Diagnostics,’ SPIE Proceedings, 4615, p.13 – 19, (2002).
J. Mobley, B. M. Cullum, M. Holland and T. Vo-Dinh, ‘Transcranial Ultrasound for Brain Injury Monitoring: Initial Clinical Studies,’ SPIE Proceedings, 4615, p.53-60 (2002).
B. M. Cullum, J. Mobley, A. L. Wintenberg, R. A. Maples, D. L. Stokes and T. Vo-Dinh, ‘Field-portable AOTF-based Monitor Technology for Environmental Sensing,’ SPIE Proceedings, 4576, p. 244-254 (2002).
T. Vo-Dinh, B. M. Cullum and D. L. Stokes, “Nanosensors and Biochips: Frontiers in Biomolecular Diagnostics,” Sensor Actuat B-Chem., 74 (1-3), p.2-11 (2001).
B. M. Cullum and T. Vo-Dinh, “Nanosensors for Biochemical Analysis of Single Cells,” Chim Oggi, 19 (1-2), p. 58-62 (2001).
S. J. Glenn, B. M. Cullum, R. B. Nair, D. A. Nivens, C. J. Murphy and S. M. Angel, “Lifetime-based Fiber-optic Water Sensor Using a Luminescent Complex in a Lithium-treated Nafion ™ Membrane,’ Analytica Chimica Acta, 448 (1-2), p. 1-8 (2001).
Z. Chi, B. M. Cullum, D. L. Stokes, J. Mobley, G. H. Miller, M. Hajaligol and T. Vo-Dinh*, “Laser-Induced Fluorescence Studies of Polycyclic Aromatic Hydrocarbon Vapors at High Temperatures,” Spectrochemica Acta A, 57 (7), p.1377-1384 (2001).
Z. Chi, B. M. Cullum, D. L. Stokes, J. Mobley, G. H. Miller, M. Hajaligol and T. Vo-Dinh, “High-temperature Vapor Detection of Polycyclic Aromatic Hydrocarbon Fluorescence,” Fuel, 80 (12), p. 1819-1824 (2001).
B. M. Cullum, G. D. Griffin, T. Vo-Dinh, ‘Nanosensors for Analysis of a Single Cell,’ SPIE Proceedings, 4254, p. 35-40 (2001).
Z. Chi, B. M. Cullum, J. Mobley, G. H. Miller and T. Vo-Dinh, ‘Vapor Detection of Polyaromatic Compounds using Laser-induced Fluorescence,’ SPIE Proceedings, 4199, p. 173-179 (2001).
J. Mobley, B. M. Cullum and T. Vo-Dinh, “Ultrasonic Diffraction in the Design of Photoacoustic Probes,” SPIE Proceedings, 4254, p. 151-163 (2001).
T. Vo-Dinh, B. M. Cullum and G. D. Griffin, “Optical Nanosensors for Single Cell Analysis,” Radiat. Res., 156(4), p. 437-438 (2001).
B. M. Cullum, J. Mobley, J. S. Bogard and T. Vo-Dinh, “Detection of Neutrons Using a Novel Three-Dimensional Optical Random Access Memory Technology (3D-ORAM),” SPIE Proceedings, 4199, p. 173-179 (2001).
T. Vo-Dinh and B. M. Cullum, “Biosensors and Biochips for Bioanalysis,” Fresen. J. Anal. Chem., 366, p. 540-551 (2000).
B. M. Cullum and T. Vo-Dinh, “Development of Optical Nanosensors for Biological Measurements,” Trends in Biotechnol., 18, p. 388- 393 (2000).
T. Vo-Dinh, G. D. Griffin, J. P. Alarie, B. M. Cullum, B. Sumpter and D. Noid, “Development of Nanosensors and Bioprobes,” J. Nanoparticle Research, 2, p. 17 (2000).
B. M. Cullum and T. Vo-Dinh, “Optical Nanosensors and Biological Measurements,” Biofutur, 205, p. A1-A6 (2000).
B. M. Cullum, J. Mobley, J. S. Bogard, M. Moscovitch, G. W. Phillips and T. Vo-Dinh, “Three-Dimensional Optical Random Access Memory (3D-ORAM) Materials and Read-out System for Radiation Dosimeters,”Anal. Chem., 72 (22), p. 5612-5617 (2000).
B. M. Cullum, G. D. Griffin, G. H. Miller and T. Vo-Dinh, “Intracellular Measurements in Mammary Carcinoma Cells Using Fiber-Optic Nanosensors,” Anal. Biochem., 277 (1), p. 25 (2000).
T. Vo-Dinh, B. M. Cullum, J. P. Alarie and G. D. Griffin, “Antibody-Based Nanosensor for Measurement in a Single Cell,” Nature Biotechnology, 18, p. 764-767 (2000).
B. M. Cullum, J. Mobley, Z. Chi, D. L. Stokes, G. H. Miller and T. Vo-Dinh, “Development of a Compact Handheld Raman Instrument with No-Moving Parts for Use in Field Analysis,” Rev. Sci. Instrum., 71, p. 1602-1607 (2000).
B. M. Cullum, Z. Chi and T. Vo-Dinh, “High Temperature Measurements and Instrumentation for Polyaromatic Hydrocarbons (PAHs): a Review,” Polycyclic Aromat. Compd., 18, p. 25-47 (2000).
P. Kasili, J. Mobley, B. M. Cullum and T. Vo-Dinh, ‘Bioimaging system using acousto-optic tunable filter,’SPIE Proceedings,  3911, p. 345-354 (2000).
J. Mobley, B. M. Cullum and T. Vo-Dinh, ‘Method for the Simultaneous Acquisition of Optical Absorption and Sonic Attenuation with Photoacoustic Ultrasound,’ SPIE Proceedings, 3911, p. 180-190 (2000).
B. M. Cullum, S. K. Shealy and S. Michael Angel, “Fiber-Optic Resonance Enhanced Multiphoton Ionization (REMPI) Probe for the In-Situ Detection of Volatile Organic Vapors,” Appl. Spectrosc., 53 (12), p. 1646-1650 (1999).
B. M. Cullum, J. Mobley, J. S. Bogard, M. Moscovitch and T. Vo-Dinh, “Development of Instrumental ORAM System for Radiation Dosimetry,” SPIE Proceedings, 3853, p. 376-384 (1999).
S. J. Glenn, B. M. Cullum, S. K. Shealy, J. M. Regal and S. M. Angel, ‘Lifetime Imaging with Optical Fibers,’SPIE Proceedings, 3860, p. 192-200 (1999).
T. Vo-Dinh, G. H. Miller, J. Mobley, B. M. Cullum and C. A. DiMarzio, “Development of Methods and Instrumentation for Spectral Imaging,” SPIE Proceedings, 3853, (1999).
S. J. Glenn, B. M. Cullum, J. C. Carter, R. B. Nair, D. A. Nivens, C. J. Murphy and S. M. Angel, “Development of a Lifetime-Based Fiber Optic Imaging Sensor to Study Water Transport in Thin Nafion Membranes,” SPIE Proceedings, 3540, p. 235-240 (1999).
B. M. Cullum, and S. M. Angel, “Development of a Fiber-Optic REMPI Probe for Environmental Contaminants,” SPIE Proceedings, 3534, p. 40-48 (1998).
R. B. Nair, B. M. Cullum and C. J. Murphy, “Optical Properties of [Ru(phen)2dppz]2+ as a Function of Nonaqueous Environment,” Inorg. Chem., 36 (6), p. 962-965 (1997).
B. J. Marquardt, B. M. Cullum, T. J. Shaw and S. M. Angel, “Fiber-Optic Probe for Determining Heavy Metals in Solids Based on Laser-Induced Plasmas,” SPIE Proceedings, 3105, p. 203-212 (1997).
K. Sooklal, B. M. Cullum, S. M. Angel, and C. J. Murphy, “Photophysical Properties of ZnS Nanoclusters with Spatially Localized Mn2+,” J. Phys. Chem., 100 (10), p. 4551-4555 (1996).

Honors and Awards

Eli Lilly Analytical Academic Award
August 5, 2004
‘SERS Nano-Imaging Probes for Real Time Chemical Imaging’
R&D 100 Award
December 1, 2003
RAMiTS: Raman Integrated Tunable Sensor
Eli Lilly Analytical Academic Award
August 5, 2003
‘Development of Implantable SERS Nanosensors’
Plenary Lecturer at Analytica Conference
April 24,2002
“Nanosensing: Molecular Detection and Monitoring at the Sub-Cellular Level”

Funding

Development of a SERS Nanoimager for Robust Biological/Chemical Signature Identification
Maryland Proof of Concept Alliance
January 2011
Project Role: PI
Center for Translational Nanobioscience: (Phase 1) Optical Nanosensors for Pre-Symptomatic Disease Monitoring
UMBC RFSI
June 2010
Project Role: PI
Innovation and Commercialization of Chemical Detection Technologies
Alex Brown Center for Entrepreneurship
March 2009
Project Role: co-PI
Multi-Layered Surround SERS Array System for the Rapid, Specific and Multiplexed Detection of Foodborne Bacteria and Toxins
USDA CSREES
January 2007
Project Role: PI
Development and Optimization of Multilayered Surface Enhanced Raman Substrates
U.S. Army Research Office
May 2005
Project Role: PI
Novel Surface Enhanced-Raman Scattering (SERS) Substrates for UV SERS
ITT Industries
January 2005
Project Role: PI
Eli Lilly Analytical Academic Award
Eli Lilly and Company
September 2004
Project Role: PI
Development of Multiphoton Photoacoustic Spectroscopy for Subsurface Tumor Diagnosis and Margining
MD TEDCO
May 2004
Project Role: PI
Development and Evaluation of Novel-Implantable Nanosensors for Real-Time Monitoring of Individual Cells and Cellular Signaling
DARPA (DSO)/ AFOSR
February 2004
Project Role: PI
Eli Lilly Analytical Academic Award
Eli Lilly and Company
September 2003
Project Role: PI

Meeting/Events

FACSS
Smart Medical and Physiological Sensor Technologies Conference
Pittcon
Pittsburgh Conference
http://www.pittcon.org

Courses Taught

  • CHEM 300: Analytical Chemistry
  • CHEM 399: Tutorial Projects in Chemistry
  • CHEM 461: Advanced Instrumental Methods of Analysis
  • CHEM 490: Special Topics in Chemistry
  • CHEM 499: Undergraduate Research